Triple FAST

SARS CoV-2 Triple Gene RT-qPCR detects SARS-CoV-2 specific ORF1ab gene, nucleocapsid (N) gene and envelope (E) gene in a single channel.


 SARS CoV-2 Triple Gene RT-qPCR detects SARS-CoV-2 specific ORF1ab gene, nucleocapsid (N) gene and envelope (E) gene in a single channel. Results are available in less than 30 minutes for improved workflow and improved patient care management. The sensitivity and specificity of SARS CoV-2 Triple Gene RT-qPCR are 100% and 100%, respectively.

The kit is applied to nucleic acid extracts from nasopharyngeal aspirate/lavage, bronchoalveolar lavage, nasopharyngeal swab, oropharyngeal swab and saliva samples using vNAT® extraction buffer or robotic extraction systems.

The kit provides rapid diagnosis by one-step reverse transcription (RT) and real-time PCR (qPCR) (RT-qPCR) method targeting N, E and RdRp gene fragments specific to SARS-CoV-2. The Oligo Mix component included in the kit also contains the human RNase-P oligo set. This set of oligos targets the exome-exome linkage in mRNA but not the human genome. In this way, sampling is used to check many conditions such as RNA integrity, nucleic acid extraction, reverse transcription and inhibition of qPCR. Contamination is ensured by the negative control included in the kit, and the stability of the qPCR reagent is controlled by the positive control.

With the kit, the RT-qPCR result can be obtained in less than 30 minutes.



Isolation systems and Real-time PCR devices for which the kit is validated



RINATM M14 Nucleic Acid Extraction System (Robot Cat No: RINA-M14-01; Consumables Cat No: RN-NA-14-111-100)

vNAT® Extraction Consumables (vNAT® Viral Nucleic Acid Buffer Cat No: BS-NA-510; vNAT® Transfer Tube Cat No: BS-NA-513-100)

Real-Time PCR*

Bio-Rad CFX96 TouchTM

Bio Molecular Systems Magnetic Induction Cycler (MIC),

Qiagen Rotor-Gene® 5 Plex

HiMedia Insta Q96® Plus



Kit's performance criteria and results

Performance Criterion



500 copies/mL (For all sample types)

Cross Reaction

Cross-reactivity with some bat-associated SARS-CoV strains based on in-silico tests







Storage temperature: (-22°C)-(-18°C); Transport temperature: 2-8°C



Content/Intended Use


Quantity (10 µl Reaction)

Birim Reaksiyon Tüketim

250 Reactions

500 Reactions

1000 Reactions

Oligo Mix


SARS-CoV-2 Detection (Orf1ab, N and E) (FAM)

1 x 625 µL

1 x 1250 µL

2 x 1250 µL

2.5 µL

Internal Control (IC) (RNase P) (HEX)

2X Prime Script Mix

DNA polymerase, dNTP mix, reaction buffer, reverse transcriptase and ribonuclease inhibitor

1 x 1250 µL

2 x 1250 µL

4 x 1250 µL

5 µL

Storage temperature: 2-8°C/-20°C; Transport temperature: 2-8°C/-20°C

If the components are frozen, after thawing for the first time, they should be stored at 2-8°C without being frozen again.


Negative Control Template

For contamination control, test at each operation.

1 x 1000 µL

1 x 1000 µL

1 x 1000 µL

2.5 µL


Positive Control Template: Synthetic SARS-CoV-2 and RnaseP genome fragment

For reagent stability check, test at each operation.

1 x 250 µL

1 x 500 µL

2 x 500µL

2.5 µL

Shelf Life 18 months: see expiry date on box. Each reagent stored at storage temperature can be used until the expiration date indicated on the tube. The expiry date of the kit is determined by the expiry date of the reagents.



250, 500, 1000 reaction

Box Size:


Catalog Number:




You can contact us for product information.

Cat#: BS2001 Ultra-optical half-skirt qPCR plate and film (96 wells)

Cat#: BS1001; Ultra Optical qPCR Strip and Cover (8 wells)